Pediatric Myeloid Leukemia-Specific miRNA Expression Profiles Induced by the Leukemic Stem Cell Niche
- To study the effect of niche mesenchymal stromal cells (MSC) exposure on microRNAs
(miR) expression in pediatric AML at different time points and compare those profiles
to the miR profiles at baseline.
- To focus on four pediatric AML-specific miRs (miR34a, miR538e, miR193e, and miR198)
which show the most significant differential expression after in vivo niche exposure at
four months (hematogenous spread).
- To determine whether altered miR expression reflects or causes the metastatic invasion
pattern of AML.
OUTLINE: Bone marrow-derived mesenchymal stromal cell (MSC) samples are implanted
subcutaneously in NOD/SCID mice. Cells are then harvested at day 0, 1 month, and 4 months
post-implantation. miRNA is isolated and analyzed by Ilumina MicroRNA Expression Profiling
single Beadchip. The obtained data is then analyzed by the Illumina Genome Studio Analysis
miR expression patterns in pediatric AML are regulated by the niche MSC-associated microenvironment
Sarah R.I. Vaiselbuh, MD
Feinstein Institute for Medical Research
United States: Federal Government