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Pediatric Myeloid Leukemia-Specific miRNA Expression Profiles Induced by the Leukemic Stem Cell Niche


N/A
N/A
30 Years
Open (Enrolling)
Both
Leukemia

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Trial Information

Pediatric Myeloid Leukemia-Specific miRNA Expression Profiles Induced by the Leukemic Stem Cell Niche


OBJECTIVES:

- To study the effect of niche mesenchymal stromal cells (MSC) exposure on microRNAs
(miR) expression in pediatric AML at different time points and compare those profiles
to the miR profiles at baseline.

- To focus on four pediatric AML-specific miRs (miR34a, miR538e, miR193e, and miR198)
which show the most significant differential expression after in vivo niche exposure at
four months (hematogenous spread).

- To determine whether altered miR expression reflects or causes the metastatic invasion
pattern of AML.

OUTLINE: Bone marrow-derived mesenchymal stromal cell (MSC) samples are implanted
subcutaneously in NOD/SCID mice. Cells are then harvested at day 0, 1 month, and 4 months
post-implantation. miRNA is isolated and analyzed by Ilumina MicroRNA Expression Profiling
single Beadchip. The obtained data is then analyzed by the Illumina Genome Studio Analysis
Software.

Inclusion Criteria


DISEASE CHARACTERISTICS:

- Diagnosis of acute myeloid leukemia

- Fresh human bone marrow samples obtained from orthopedic surgery at the Tissue
Donation Program of The Feinstein Institute and the Children Oncology Group (COG)
Myeloid Disease Biorepository

PATIENT CHARACTERISTICS:

- Not specified

PRIOR CONCURRENT THERAPY:

- Not specified

Type of Study:

Observational

Study Design:

N/A

Outcome Measure:

miR expression patterns in pediatric AML are regulated by the niche MSC-associated microenvironment

Safety Issue:

No

Principal Investigator

Sarah R.I. Vaiselbuh, MD

Investigator Role:

Principal Investigator

Investigator Affiliation:

Feinstein Institute for Medical Research

Authority:

United States: Federal Government

Study ID:

CDR0000695579

NCT ID:

NCT01298414

Start Date:

February 2011

Completion Date:

Related Keywords:

  • Leukemia
  • childhood acute myeloid leukemia/other myeloid malignancies
  • Leukemia
  • Leukemia, Myeloid, Acute
  • Leukemia, Myeloid

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