Trial Information
Somatic Mutations in Stem and Progenitor Cells in AML
OBJECTIVES:
- To rigorously examine fractionated hematopoietic stem and progenitor cells from
patients with acute myeloid leukemia (AML).
- To define somatic mutations occurring in de novo AML, including the FLT3-ITD, CEBPA,
NPM1, TET2, ASXL1, IDH1, and IDH2 mutations.
- To examine what cellular compartments (LT-HSC, ST-HSC, CMP, GMP, and MEP) contain the
selected mutations and in what order those mutations occur.
OUTLINE: Archived bone marrow specimens are analyzed for sequencing and detection of
mutations by Sanger-based resequencing of PCR amplicons. Results are then compared with the
results of age-matched healthy controls.
Inclusion Criteria
DISEASE CHARACTERISTICS:
- Diagnosis of acute myeloid leukemia
- Bone marrow samples must meet 1 of the following criteria:
- Normal karyotype from patients enrolled on ECOG-1900 archived at the ECOG
Leukemia Tissue Bank
- Live, sortable mononuclear cells prepared at the time of initial diagnosis
(slow-frozen in media with 10% DMSO OR fresh cells shipped immediately
- Age-matched healthy control samples from commercial providers
PATIENT CHARACTERISTICS:
- Not specified
PRIOR CONCURRENT THERAPY:
- Not specified
Type of Study:
Observational
Study Design:
N/A
Outcome Measure:
Define somatic mutations occurring in de novo AML
Safety Issue:
No
Principal Investigator
Ulrich G. Steidl, MD, BS
Investigator Role:
Principal Investigator
Investigator Affiliation:
Albert Einstein College of Medicine of Yeshiva University
Authority:
Unspecified
Study ID:
CDR0000688223
NCT ID:
NCT01251159
Start Date:
September 2010
Completion Date:
Related Keywords:
- Leukemia
- adult acute myeloid leukemia with 11q23 (MLL) abnormalities
- adult acute myeloid leukemia with del(5q)
- adult acute myeloid leukemia with inv(16)(p13;q22)
- adult acute myeloid leukemia with t(16;16)(p13;q22)
- adult acute myeloid leukemia with t(8;21)(q22;q22)
- untreated adult acute myeloid leukemia
- adult acute minimally differentiated myeloid leukemia (M0)
- adult acute myeloblastic leukemia with maturation (M2)
- adult acute myeloblastic leukemia without maturation (M1)
- adult acute myelomonocytic leukemia (M4)
- adult acute monoblastic leukemia (M5a)
- adult acute monocytic leukemia (M5b)
- adult erythroleukemia (M6a)
- adult pure erythroid leukemia (M6b)
- adult acute megakaryoblastic leukemia (M7)
- Leukemia
- Leukemia, Myeloid, Acute
- Leukemia, Myeloid