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Somatic Mutations in Stem and Progenitor Cells in AML


N/A
16 Years
60 Years
Not Enrolling
Both
Leukemia

Thank you

Trial Information

Somatic Mutations in Stem and Progenitor Cells in AML


OBJECTIVES:

- To rigorously examine fractionated hematopoietic stem and progenitor cells from
patients with acute myeloid leukemia (AML).

- To define somatic mutations occurring in de novo AML, including the FLT3-ITD, CEBPA,
NPM1, TET2, ASXL1, IDH1, and IDH2 mutations.

- To examine what cellular compartments (LT-HSC, ST-HSC, CMP, GMP, and MEP) contain the
selected mutations and in what order those mutations occur.

OUTLINE: Archived bone marrow specimens are analyzed for sequencing and detection of
mutations by Sanger-based resequencing of PCR amplicons. Results are then compared with the
results of age-matched healthy controls.

Inclusion Criteria


DISEASE CHARACTERISTICS:

- Diagnosis of acute myeloid leukemia

- Bone marrow samples must meet 1 of the following criteria:

- Normal karyotype from patients enrolled on ECOG-1900 archived at the ECOG
Leukemia Tissue Bank

- Live, sortable mononuclear cells prepared at the time of initial diagnosis
(slow-frozen in media with 10% DMSO OR fresh cells shipped immediately

- Age-matched healthy control samples from commercial providers

PATIENT CHARACTERISTICS:

- Not specified

PRIOR CONCURRENT THERAPY:

- Not specified

Type of Study:

Observational

Study Design:

N/A

Outcome Measure:

Define somatic mutations occurring in de novo AML

Safety Issue:

No

Principal Investigator

Ulrich G. Steidl, MD, BS

Investigator Role:

Principal Investigator

Investigator Affiliation:

Albert Einstein College of Medicine of Yeshiva University

Authority:

Unspecified

Study ID:

CDR0000688223

NCT ID:

NCT01251159

Start Date:

September 2010

Completion Date:

Related Keywords:

  • Leukemia
  • adult acute myeloid leukemia with 11q23 (MLL) abnormalities
  • adult acute myeloid leukemia with del(5q)
  • adult acute myeloid leukemia with inv(16)(p13;q22)
  • adult acute myeloid leukemia with t(16;16)(p13;q22)
  • adult acute myeloid leukemia with t(8;21)(q22;q22)
  • untreated adult acute myeloid leukemia
  • adult acute minimally differentiated myeloid leukemia (M0)
  • adult acute myeloblastic leukemia with maturation (M2)
  • adult acute myeloblastic leukemia without maturation (M1)
  • adult acute myelomonocytic leukemia (M4)
  • adult acute monoblastic leukemia (M5a)
  • adult acute monocytic leukemia (M5b)
  • adult erythroleukemia (M6a)
  • adult pure erythroid leukemia (M6b)
  • adult acute megakaryoblastic leukemia (M7)
  • Leukemia
  • Leukemia, Myeloid, Acute
  • Leukemia, Myeloid

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