Regulation of S100A10 by the PML-RAR-alpha Oncoprotein
- Demonstrate that S100A10 is expressed on APL cells and that levels of S100A10
correspond to the amount of fibrinolytic activity.
- Evaluate the impact of ATRA on S100A10 level and fibrinolytic activity in vitro.
- Evaluate the impact of S100A10 knockdown on fibrinolytic activity.
- Correlate in vitro findings in cell culture to primary patient samples both pre- and
OUTLINE: This is a multicenter study.
Previously collected samples are analyzed via flow cytometry and western blot analysis. Cell
surface levels of S100A10 and annexin A2 are correlated to the promyelocyte population and
total protein levels are examined to determine total annexin A2 and S100A10 in acute
promyelocytic leukemia cells.
Demonstration that S100A10 is expressed on acute promyelocytic leukemia cells and that levels of S100A10 correspond to the amount of fibrinolytic activity
Jason N. Berman, MD
IWK Health Centre
United States: Federal Government