Gene Expression Profiling of Metastatic Breast Cancer Predict the Therapeutic Response to Chemotherapy
1. Metastatic breast cancer tissue is obtained from core needle biopsies pretreatment and
is flash frozen and stored at -70℃ until processing.
2. After patients received docetaxel combination thiotepa for two cycles, response is
assessed using Response Evaluation Criteria in Solid Tumor Group (RECIST) guidelines.
Degree of response is used to divide the cancers into two groups—sensitive and
3. The gene expression in metastatic breast cancer tissue sample is detected by microarray
to screen gene markers that are differently expressed between groups
4. Statistical analysis is performed using unsupervised hierarchical cluster.
Observational Model: Cohort, Time Perspective: Prospective
Response to chemotherapy is assessed using Response Evaluation Criteria in Solid Tumor Group (RECIST) guidelines
Zheng Wang, PhD
Beijing Cancer Hospital
China: Ministry of Health