Comparison of Bone Morphogenetic Protein-4 and -7 Levels (BMP-4 and 7) in the Serum, Ovarian Follicle Aspirates and Ovarian Organ Culture Supernatants Between Healthy Controls and Patients With Polycystic Ovarian Syndrome (PCOS).
Female patients at ages 20-35 diagnosed with polycystic ovarian syndrome and age-matched
healthy control subjects will be enrolled in this study.
Blood samples will be obtained at early follicular phase during doctor's visit from PCOS
patients and control subjects.If the patient is amenorrheic at the time of visit, both
random blood samples and another sample at early follicular phase after induction of
withdrawal bleeding with progesterone.
Follicular fluids will be retrieved during oocyte pick-up procedure in patients undergoing
assisted reproduction for PCOS and control IVF patients whose infertility is not PCO
Samples from ovaries will be collected at operation from PCOS patients and control subjects
if they already undergo any gynecologic laparotomi operations.
Collected samples will be cultured for 7 days and BMP-4 and -7 levels will be measured in
the culture fluid of the samples using ELISA method.
Observational Model: Case Control, Time Perspective: Cross-Sectional
Bulent Urman, MD
VKV American Hospital Women's Health center
Turkey: Ministry of Health