Epigenetic Alterations in AML
- Map key histone modifications and cytosine methylation in leukemia cell lines with
defined oncogenic mutations.
- Acquire genome-wide maps of key histone modifications and cytosine methylation for
primary leukemia in samples from patients with acute myeloid leukemia (AML).
- Investigate functional relationships between genetic and epigenetic landscapes in AML.
OUTLINE: Archived samples are analyzed in vivo and in vitro (cell line) for histone
modification and cytosine methylation. Genome-wide locations of 5 histone modifications are
analyzed using chip-Seq, as well as DNA methylation analysis at nucleotide-resolution by
high-throughput bisulfite sequencing. These epigenetic data are correlated with genomic data
(sequence analysis, expression array, SNP array).
Relationships between genetic and epigenetic landscapes in AML
Soheil Meshinchi, MD
Fred Hutchinson Cancer Research Center
United States: Federal Government