Observe Change of Endotoxaemia and Related Mediators in Patients With Chronic HBV Infection
1. Patients Patients with chronic HBV infection and healthy volunteers were enrolled.
There were asymptomatic carriers group, patients with chronic hepatitis, patients with
hepatic cirrhosis, and patients with acute on chronic liver failure.
2. Endotoxin assay Blood samples were obtained under aseptic conditions by peripheral
venipuncture and using pyrogen-free syringes, needles, and glassware. Plasma samples
were heated at 70℃ for 10 min. Plasma concentration was then measured in duplicate
using a commercially available Limulus amebocyte lysate assay following the
3. ELISA ELISA kits were used to assess in duplicate the plasma concentrations of IL-6,
IL-10, TNFα, sICAM-1 and sCD14.
Observational Model: Cohort, Time Perspective: Prospective
plasma levels of endotoxin
Gao Zhiliang, Professor
The Third Affliated Hospital of Sun Yat-sen University
China: Food and Drug Administration