Critical Role of MicroRNA-34a and MicroRNA-194 in Acute Myeloid Leukemia With CEBPA Mutations
- To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα
during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations.
- To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid
progenitors with C/EBPα mutations leads to granulocytic differentiation.
- To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis.
- To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation.
OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including
mutation analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell
sorting (FACS) analysis.
Significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis
Soheil Meshinchi, MD
Fred Hutchinson Cancer Research Center
United States: Federal Government