Trial Information

Allogeneic Hematopoietic Cell Transplantation for Patients With Acute Lymphoblastic Leukemia in Remission Using HLA-matched Sibling Donors, HLA-matched Unrelated Donors, or HLA-mismatched Familial Donors-A Phase 2 Study

1.0 STUDY OBJECTIVES

1.1. To evaluate the efficacy of allogeneic hematopoietic cell transplantation (HCT) in
patients with acute lymphoblastic leukemia (ALL) either who have achieved complete remission
(CR) after induction chemotherapy or who experienced recurrent leukemia then achieved second
CR after salvage chemotherapy. The efficacy of the treatment will be measured in terms of
the frequency of relapse after HCT and the duration of remission (the primary endpoints).

1.2. The secondary end points of the study include; engraftment (absolute neutrophil count
over 500/㎕, unsupported platelet count over 20,000/㎕), donor chimerism at 2 and 4 weeks
after HCT, secondary graft failure, acute and chronic graft-versus-host disease (GVHD),
lymphocyte subset recovery at 1, 2, 3, 6, and 12 months, cytomegalovirus (CMV)
reactivation/CMV disease, Epstein-Barr virus (EBV) reactivation/post-transplant
lymphoproliferative disorder, transplantation-related mortality (100 day, 1 year), leukemia
free survival, and overall survival.

1. 3. The hematopoietic cell donors in the study will include; HLA-matched sibling donors,
HLA-matched unrelated donors, and HLA-mismatched family members, so that the majority of
patients who achieve CR after induction or salvage chemotherapy will undergo allogeneic HCT
as a part of consolidation therapy.

2.0 BACKGROUND INFORMATION

2.1. ALL is a malignant disorder characterized by the rapid proliferation of immature
lymphocytes, which results in the accumulation and infiltration of neoplastic lymphocytes in
the blood/bone marrow and other tissues. Allogeneic HCT is a curative therapeutic modality
for a significant proportion of patients with ALL. Allogeneic HCT from HLA-matched sibling
donors can produced long-term leukemia free survival in patients with ALL in high-risk first
CR or second CR.(1) In adults with standard-risk ALL, the greatest benefit is achieved from
a matched sibling allogeneic transplantation when compared to autologous transplantation or
consolidation/maintenance therapy in the first CR status.(2)

2.2. Wider application of allogeneic HCT in patients with ALL, however, is impeded by
limited donor availability. Less than a third of patients who need allogeneic HCT will have
a HLA-matched sibling who can donate hematopoietic cells. For those patients who do not have
an HLA-matched donor in the family, provided that they do not carry rare or private
HLA-haplotype, HLA-matched unrelated donor can be found.(3) The chance of finding a willing
unrelated donor in Korea is about 50%. On the other hand, nearly all patients who are in the
need of allogeneic HCT have at least one HLA-haploidentical familial member, who is most
willing to give hematopoietic cells immediately, not only for the initial transplantation,
but also for the subsequent additional donations, if those became necessary. Early attempts
to transplant allogeneic hematopoietic cells across the barriers of HLA-haplotype difference
was met with high frequencies of engraftment failure and severe graft-versus-host disease
(GVHD).(4, 5) Depletion of donor T cells from the graft before HCT decreased the frequency
and severity of GVHD. However, it resulted in increased graft failure, delayed immune
reconstitution, and increased fatal infections.(6-8) Further efforts to improve the outcomes
of HLA-mismatched familial donor HCT included use of polyclonal(9, 10) or monoclonal
antibodies(11, 12) against T cell as a part of conditioning regimen (in vivo-T cell
depletion) and incorporation of the concept of feto-maternal immune tolerance in the donor
selection process among several available HLA-haploidentical family members.(13)

2.3. In addition to aforementioned approaches, use of RIC in the setting of HLA-mismatched
familial donor HCT has been explored. Various RIC regimens, utilizing total body irradiation
(TBI),(14, 15) busulfan,(16, 17) or melphalan,(18) along with fludarabine, have been shown
to be effective in achieving successful engraftment with less transplantation-related
mortality (TRM), especially in elderly patients and in patients with organ dysfunctions, in
the setting of HLA-matched donor HCT. These findings showed that under adequate
immunosuppression, but not necessarily myeloablation, of the patients, donor hematopoietic
cells can engraft and complete donor hematopoietic chimerism can be achieved. There are data
that suggest the same principle may be extended to HLA-mismatched HCT settings as well.
Successful engraftment of allogeneic hematopoietic cells across HLA-haplotype difference has
been well-documented after RIC in animal models,(19-21) and in infants with severe combined
immunodeficiency syndrome.(22, 23) In adult patient with hematologic malignancies, several
studies involving small number of patients showed feasibility of successful engraftment of
hematopoietic cell graft from HLA-haploidentical familial donor after RIC.(24-27)

2.4. Data generated in our hospital enhance the evidence of feasibility of hematopoietic
engraftment across the HLA-haplotype barrier in adult patients after RIC.(28) Between April
2004 and February 2008, 31 patients (including 21 patients with acute leukemia) underwent
HLA-haploidentical HCT after RIC of busulfan, fludarabine, and ATG and all 28 evaluable
patients achieved engraftment with absolute lymphocyte count (ANC) over 500/㎕ on median day
16.5. As early as 2 weeks after HCT, 22 of 24 evaluated patients showed complete donor
chimerism of 95% or over. None of the patients in the study experienced secondary graft
failure. While achieving consistent and durable donor cell engraftment, the cumulative
incidences of grades 2-4 acute and chronic GVHD were 19%, and 20%, respectively.

2.5. Between May 2008 and May 2009, 31 additional patients with acute leukemia were treated
with HLA-mismatched HCT using the same treatment strategy as in the aforementioned study. As
such, the data of 52 patients are now available. There were 24 male and 28 female with
median age of 39.5 years (range, 16-70). Thirty-seven patients had AML, 13 ALL, and 2 acute
mixed lineage leukemia. Ten patients were in first CR status, 15 in second or third CR
status, and 27 had refractory disease. The donors were either offsprings (n=23), mothers
(n=16), or siblings (n=13) of the patients and their median age was 37 years (range, 3-68).
The conditioning regimen for HCT included busulfan in reduced-dose, fludarabine, and
antithymocyte globulin. GVHD prophylaxis was given with cyclosporine and methotrexate. Other
than 4 patients who experienced leukemia progression within 30 days of HCT or died early,
all the rest 48 patients achieved donor cell engraftment with absolute neutrophil count
(ANC) >500/㎕ on median 14.5 days (range, 10-27). One patient experienced secondary graft
failure subsequently. Cumulative incidence rates for acute GVHD grade 2-4 and chronic GVHD
were 10% (95% CI, 4%-23%) and 33% (95% CI, 22%-51%), respectively. Cumulative incidence
rates of leukemia progression/recurrence were 13%, 41%, and 77% for patients in CR1, CR2-3,
and refractory leukemia at the time of HCT. In all, five patients in the series died without
leukemia progression/recurrence giving transplantation-related mortality (TRM) rate of 12%
(95% CI, 5%-29%). Kaplan-Meier event-free survival and overall survival rates were 44% and
50% for patients in CR1 at HCT, 40% and 23% for patients in CR2-3, and 10% and 15% for
patients with refractory leukemia. These results showed that HCT from an HLA-mismatched
family member can be performed in patients with acute leukemia successfully without
increased GVHD or TRM.

2.6. In our current prospective study, we try to integrate HLA-mismatched HCT in overall
care of patients with ALL in the first or second CR. In the past, those patients without an
HLA-matched donor in the family or unrelated donor registry were not offered allogeneic HCT.
The outcomes of HCT will be analyzed according to several clinical variables such as
patient, disease, and donor characteristics.