Study of Biomarkers in Tissue Samples of Patients With Stage III Colon Cancer Treated With Adjuvant Chemotherapy Comprising Fluorouracil-Based Regimens(B-CAST)
- Identify predictive factors of efficacy and safety of adjuvant chemotherapy comprising
fluorouracil-based regimens in patients with stage III colon cancer.
- Investigate possibility of "personalized treatment" with adjuvant chemotherapy based on
the predictive factors identified.
OUTLINE: Patients' tumor tissue samples are collected for protein expression levels of
thymidine phosphorylase (TP), dihydropyrimidine dehydrogenase (DPD), VEGF, EGFR,
mRNA-expression levels of TP, DPD, thymidylate synthase (TS), and orotate phosphoribosyl
transferase (OPRT) by ELISA, Human Duo Set, and RT-PCR assays.
Patients' enzyme expression levels are compared with their clinical data.
Observational Model: Cohort, Time Perspective: Prospective
Relationship between expression levels of enzymes and disease-free survival, relapse-free survival, and overall survival
5 years after the end of registration
Kenichi Sugihara, MD, PhD
Tokyo Medical and Dental University
Japan: Institutional Review Board, Tokyo Medical and Dental University