Assessment of Patterns of Expression of VEGF, Matrix Metalloproteinase 2, 9 Activity, E-Cadherin Regulation and CA-9 Activity as Predictors of Aggressive Renal Cell Carcinoma Behavior
OBJECTIVES:
- To identify consistent patterns of VEGF expression, E-cadherin regulation, matrix
metalloproteinase 2 and 9 activity, and carbonic anhydrase 9 activity as predictive
factors in the development of aggressive tumor behavior in tumor tissue samples from
patients with localized clear cell renal cell carcinoma.
OUTLINE: Previously collected tumor tissue samples are examined by immunohistochemistry for
levels of expression of five different factors related to aggressive tumor behavior (i.e.,
VEGF expression, E-cadherin regulation, matrix metalloproteinase 2 and 9 activity, and
carbonic anhydrase 9 activity). Samples are compared to positive tissue controls (i.e.,
VEGF- tonsil; E- cadherin-normal breast or endometrium; MMP2- placenta) and a negative
control (i.e., isotype matched normal serum) to identify consistent patterns of
expression/activity of these factors. Levels of expression are quantified as 5-level scores
(0 to 4 scale) and differences between any two areas of a tumor are analyzed.
Observational
Observational Model: Case Control, Time Perspective: Prospective
Relative patterns of expression/activity of five different growth factors/enzymes inherently related to the development of metastatic renal cell carcinoma behavior
No
David A. Levy, MD
Principal Investigator
Ireland Cancer Center at University Hospitals Case Medical Center, Case Comprehensive Cancer Center
United States: Federal Government
CASE1808
NCT00908739
May 2008
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