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Comparison of P-gp Efflux and Accumulation Assay Results With ECOG 3999 Leukemia Samples


N/A
18 Years
N/A
Not Enrolling
Both
Leukemia

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Trial Information

Comparison of P-gp Efflux and Accumulation Assay Results With ECOG 3999 Leukemia Samples


OBJECTIVES:

- Ascertain whether assessments of P-glycoprotein (P-gp) status using the accumulation
assay find greater than 10% positive specimens among 30 specimens (collected from
patients with acute myeloid leukemia enrolled on clinical trial ECOG-E3999) found to be
negative using only the efflux assay.

OUTLINE: This is a multicenter study.

Cryopreserved bone marrow specimens are examined for P-glycoprotein (P-gp) by the
accumulation assay and the efflux assay using DiOC_2 dye. Flow cytometry is used for
measuring activity in both assays.

PROJECTED ACCRUAL: A total of 40 specimens will be accrued for this study.

Inclusion Criteria


DISEASE CHARACTERISTICS:

- Cryopreserved bone marrow specimens collected from patients with acute myeloid
leukemia enrolled on clinical trial ECOG-E3999 meeting the following criteria:

- Appreciable levels of either CD34+ OR CD117+ blasts

- Appreciable staining with anti-P-gp antibodies

- 30 specimens must exhibit low to moderate dye loading for the Rh123 efflux assay

- 10 specimens must exhibit positive Rh123 efflux activities

PATIENT CHARACTERISTICS:

- Not specified

PRIOR CONCURRENT THERAPY:

- Not specified

Type of Study:

Observational

Study Design:

N/A

Outcome Measure:

Percentage of specimens collected from patients that are found to be P-glycoprotein (P-gp)-positive by accumulation assay that were found to be negative using efflux assay

Safety Issue:

No

Principal Investigator

Elisabeth Paietta, PhD

Investigator Role:

Study Chair

Investigator Affiliation:

Our Lady of Mercy Medical Center Comprehensive Cancer Center

Authority:

Unspecified

Study ID:

CDR0000478869

NCT ID:

NCT00900380

Start Date:

Completion Date:

Related Keywords:

  • Leukemia
  • adult acute monocytic leukemia (M5b)
  • adult acute megakaryoblastic leukemia (M7)
  • adult acute myeloblastic leukemia with maturation (M2)
  • adult acute myeloblastic leukemia without maturation (M1)
  • adult acute myelomonocytic leukemia (M4)
  • adult acute monoblastic leukemia (M5a)
  • secondary acute myeloid leukemia
  • untreated adult acute myeloid leukemia
  • adult acute minimally differentiated myeloid leukemia (M0)
  • adult acute myeloid leukemia with 11q23 (MLL) abnormalities
  • adult acute myeloid leukemia with inv(16)(p13;q22)
  • adult acute myeloid leukemia with t(16;16)(p13;q22)
  • adult acute myeloid leukemia with t(8;21)(q22;q22)
  • adult erythroleukemia (M6a)
  • adult pure erythroid leukemia (M6b)
  • Leukemia
  • Leukemia, Myeloid, Acute
  • Leukemia, Myeloid

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