Trial Information
Comparison of P-gp Efflux and Accumulation Assay Results With ECOG 3999 Leukemia Samples
OBJECTIVES:
- Ascertain whether assessments of P-glycoprotein (P-gp) status using the accumulation
assay find greater than 10% positive specimens among 30 specimens (collected from
patients with acute myeloid leukemia enrolled on clinical trial ECOG-E3999) found to be
negative using only the efflux assay.
OUTLINE: This is a multicenter study.
Cryopreserved bone marrow specimens are examined for P-glycoprotein (P-gp) by the
accumulation assay and the efflux assay using DiOC_2 dye. Flow cytometry is used for
measuring activity in both assays.
PROJECTED ACCRUAL: A total of 40 specimens will be accrued for this study.
Inclusion Criteria
DISEASE CHARACTERISTICS:
- Cryopreserved bone marrow specimens collected from patients with acute myeloid
leukemia enrolled on clinical trial ECOG-E3999 meeting the following criteria:
- Appreciable levels of either CD34+ OR CD117+ blasts
- Appreciable staining with anti-P-gp antibodies
- 30 specimens must exhibit low to moderate dye loading for the Rh123 efflux assay
- 10 specimens must exhibit positive Rh123 efflux activities
PATIENT CHARACTERISTICS:
- Not specified
PRIOR CONCURRENT THERAPY:
- Not specified
Type of Study:
Observational
Study Design:
N/A
Outcome Measure:
Percentage of specimens collected from patients that are found to be P-glycoprotein (P-gp)-positive by accumulation assay that were found to be negative using efflux assay
Safety Issue:
No
Principal Investigator
Elisabeth Paietta, PhD
Investigator Role:
Study Chair
Investigator Affiliation:
Our Lady of Mercy Medical Center Comprehensive Cancer Center
Authority:
Unspecified
Study ID:
CDR0000478869
NCT ID:
NCT00900380
Start Date:
Completion Date:
Related Keywords:
- Leukemia
- adult acute monocytic leukemia (M5b)
- adult acute megakaryoblastic leukemia (M7)
- adult acute myeloblastic leukemia with maturation (M2)
- adult acute myeloblastic leukemia without maturation (M1)
- adult acute myelomonocytic leukemia (M4)
- adult acute monoblastic leukemia (M5a)
- secondary acute myeloid leukemia
- untreated adult acute myeloid leukemia
- adult acute minimally differentiated myeloid leukemia (M0)
- adult acute myeloid leukemia with 11q23 (MLL) abnormalities
- adult acute myeloid leukemia with inv(16)(p13;q22)
- adult acute myeloid leukemia with t(16;16)(p13;q22)
- adult acute myeloid leukemia with t(8;21)(q22;q22)
- adult erythroleukemia (M6a)
- adult pure erythroid leukemia (M6b)
- Leukemia
- Leukemia, Myeloid, Acute
- Leukemia, Myeloid