Trial Information
Determining the Clinicopathological Significance of CaMKIIgamma Activation in AML
OBJECTIVES:
- Determine whether calmodulin-dependent protein kinase II-γ (CaMKIIγ) activation
(autophosphorylation) is associated with specific clinicopathological variables and
outcomes in blood and bone marrow samples from patients with previously untreated
primary acute myeloid leukemia.
OUTLINE: Blood and bone marrow samples are analyzed by western blot using SDS-PAGE and
immunoblotting using anti-calmodulin-dependent protein kinase II-γ (CaMKIIγ) and
anti-phosphospecific (Thr286, 287) CaMKIIγ antibodies with signals detected via
chemiluminescence. CaMKIIγ activation levels are linked to clinical variables within the
SWOG database.
Inclusion Criteria
DISEASE CHARACTERISTICS:
- Cryopreserved specimens of marrow or peripheral blood from patients enrolled in
clinical trial SWOG-9333 and meeting the following criteria:
- Diagnosis of primary acute myeloid leukemia
- No M3 disease
- Previously untreated disease
- Randomized to remission induction chemotherapy with mitoxantrone hydrochloride
and etoposide (Arm II)
PATIENT CHARACTERISTICS:
- Not specified
PRIOR CONCURRENT THERAPY:
- See Disease Characteristics
Type of Study:
Observational
Study Design:
Time Perspective: Retrospective
Outcome Measure:
Association of specific clinicopathological variables and outcomes with calmodulin-dependent protein kinase II-γ (CaMKIIγ) activation (autophosphorylation)
Outcome Time Frame:
upon receipt of specimens
Safety Issue:
No
Principal Investigator
Steven J. Collins, MD
Investigator Role:
Principal Investigator
Investigator Affiliation:
Fred Hutchinson Cancer Research Center
Authority:
United States: Federal Government
Study ID:
CDR0000612640
NCT ID:
NCT00899743
Start Date:
August 2008
Completion Date:
June 2009
Related Keywords:
- Leukemia
- adult erythroleukemia (M6a)
- adult pure erythroid leukemia (M6b)
- adult acute megakaryoblastic leukemia (M7)
- adult acute minimally differentiated myeloid leukemia (M0)
- adult acute monoblastic leukemia (M5a)
- adult acute monocytic leukemia (M5b)
- adult acute myeloblastic leukemia with maturation (M2)
- adult acute myeloblastic leukemia without maturation (M1)
- adult acute myelomonocytic leukemia (M4)
- adult acute myeloid leukemia with t(8;21)(q22;q22)
- adult acute myeloid leukemia with t(16;16)(p13;q22)
- adult acute myeloid leukemia with inv(16)(p13;q22)
- adult acute myeloid leukemia with 11q23 (MLL) abnormalities
- untreated adult acute myeloid leukemia
- Leukemia
- Leukemia, Myeloid, Acute
- Leukemia, Myeloid