Molecular and Functional Characterisation of Bone Marrow Function in Normal Subjects, Myelodysplastic Syndromes (MDS) and Secondary Disorders of Heamatopoiesis
- To identify novel biomarkers of disease that would help in the initial diagnosis of
myelodysplastic syndromes (MDS).
- To understand the genesis of anemia in cancers.
- To identify novel biomarkers of disease that predict progression of MDS to acute
OUTLINE: Blood and bone marrow samples are collected. Hemopoietic stem cells (HSC) and
progenitor cells are isolated from samples for analysis. Some of these HSC and progenitor
cells are used for functional assays. From the rest of the cells, DNA, RNA, and protein is
extracted for molecular analyses, including gene mutation analysis, gene methylation assays,
chromatin immunoprecipitation, microarray, and real-time polymerase chain reaction.
Identification of novel biomarkers of disease
Vyas Paresh, MD
Oxford Radcliffe Hospital