Antioxidant Enzymes and Oxidative DNA Damage in Subjects With 'High-Risk' for Prostate Cancer
- To determine whether there are any changes in pro-oxidant-antioxidant profiles in
patients who are at high risk for developing prostate cancer.
- To compare the profiles of these patients with those of healthy controls.
OUTLINE: Participants undergo blood sample collection. Samples are used to measure levels of
antioxidant enzymes (i.e., glutathione, glutathione peroxidase, glutathione reductase,
superoxide dismutase, and reduced glutathione) and biomarkers of oxidative stress and DNA
damage in leukocytes (i.e., thiobarbituric acid reactive substances [TBARS] and
8-hydroxydeoxyguanosine). Samples are analyzed via antioxidant enzyme assay, TBARS assay,
and enzyme-linked immunosorbent assay.
PROJECTED ACCRUAL: A total of 40 participants (20 at high risk for developing prostate
cancer and 20 healthy controls) will be accrued for this study.
Observational Model: Case Control, Time Perspective: Prospective
Plasma levels of antioxidant enzymes as assessed by antioxidant enzyme assay, TBARS assay, and enzyme-linked immunosorbent assay
Blood (5ml) will be drawn from subjects for use in the various assays.
at time of study entry
Sanjay Gupta, PhD
Case Medical Center, University Hospitals Seidman Cancer Center, Case Comprehensive Cancer Center
United States: Institutional Review Board
|Case Medical Center, University Hospitals Seidman Cancer Center, Case Comprehensive Cancer Center||Cleveland, Ohio 44106-5065|
|UHHS Chagrin Highlands Medical Center||Orange Villager, Ohio 44122|
|University Suburban Health Center||South Euclid, Ohio 44121|