Genetic Risk Classes in Adult Acute Lymphocytic Leukemia
- Identify genes involved in specific biologic processes or molecular functions that
contribute to the mechanisms by which the BCR/ABL tyrosine kinase induces a leukemic
phenotype using RNA banked from patients with BCR/ABL-positive acute lymphoblastic
leukemia (ALL) enrolled on ECOG-2993.
- Compare patterns of mRNA expression of BCR/ABL fusion protein in patients with
B-lineage ALL vs patients with ALL and no cytogenetic abnormalities enrolled on
- Determine both shared and differing expression patterns in patients with
BCR/ABL-positive and cytogenetically negative ALL with respect to achievement of
complete remission and duration of disease-free and overall survival.
OUTLINE: This is a multicenter study.
Total RNA is isolated from stored tissue samples and integrity is verified by reverse
transcription-polymerase chain reaction (RT-PCR). cDNA libraries are created from total RNA
and gene expression is analyzed via microarray analysis.
Genes of interest are further analyzed by flow cytometry and RT-PCR.
PROJECTED ACCRUAL: A total of 137 patients will be accrued for this study.
Genes involved in specific biologic processes or molecular functions that contribute to the mechanisms by which the BCR/ABL tyrosine kinase induces a leukemic phenotype
Elisabeth Paietta, PhD
Our Lady of Mercy Medical Center Comprehensive Cancer Center