Trial Information

Prognostic Value of p53 and/or p16 Alterations in Ewing Sarcoma

OBJECTIVES:

Primary

- Determine if mutation of p53, and/or deletion of the p16 locus, have prognostic value
in patients with Ewing sarcoma.

Secondary

- Estimate the incidence of p53 mutation in Ewing sarcoma samples collected from COG
studies.

- Estimate the incidence of p16 deletions in Ewing sarcoma samples collected from COG
studies.

- Prepare and archive amplified genomic DNA from Ewing sarcoma samples collected from COG
studies for future biologic analysis.

OUTLINE: This is a multicenter study.

Previously archived tumor samples are analyzed for p53 mutations and p16 deletion by
immunohistochemistry, FISH, PCR, and DNA sequencing.