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Isolation and Identification of Lung Cancer Precursor Cells From Malignant Pleural Effusion Specimens


N/A
N/A
N/A
Not Enrolling
Both
Lung Cancer, Metastatic Cancer

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Trial Information

Isolation and Identification of Lung Cancer Precursor Cells From Malignant Pleural Effusion Specimens


OBJECTIVES:

- Isolate and identify lung cancer precursor cells in discarded pleural fluid specimens
obtained during routine thoracentesis procedures for the diagnosis and treatment of
malignant pleural effusions.

OUTLINE: Malignant pleural effusion specimens obtained from the Pathology Department at the
University Hospitals of Cleveland are analyzed using an immunomagnetic bead enrichment assay
to isolate cells of epithelial origin. The isolated cells are then stained with fluorescent
DNA binding dye, Hoechst 33342, and other markers (i.e., CD133, CD24/CD44) and sorted by
flow cytometry into progenitor and nonprogenitor subpopulations. The sorted subpopulations
are separated and examined functionally by in vitro and in vivo studies. Sphere culture
studies are also performed on the sorted subpopulations. The specimens may also be utilized
for transcriptional profiling studies, RNAi studies, and for the establishment of long-term
cultures and cell lines. DNA is also isolated and stored from tumor and nonepithelial normal
cell compartments for future genetic studies on known and unidentified genetic abnormalities
contributing to the development of lung cancer, such as EGFR, ErbB2, K-ras, and p53
mutational status.

Inclusion Criteria


DISEASE CHARACTERISTICS:

- Cytologically positive malignant pleural effusion secondary to primary small cell or
non-small cell lung cancer

- Recruited from the Division of Hematology/Oncology, the Multidisciplinary Thoracic
Oncology Group of Ireland Cancer Center, or the inpatient or outpatient units of Case
Medical Center-University Hospitals

PATIENT CHARACTERISTICS:

- Not specified

PRIOR CONCURRENT THERAPY:

- Not specified

Type of Study:

Observational

Study Design:

Time Perspective: Retrospective

Outcome Measure:

Isolation and identification of lung cancer precursor cells in malignant pleural effusion specimens

Safety Issue:

No

Principal Investigator

Balazs Halmos, MD

Investigator Role:

Principal Investigator

Investigator Affiliation:

Case Medical Center, University Hospitals Seidman Cancer Center, Case Comprehensive Cancer Center

Authority:

United States: Institutional Review Board

Study ID:

CASE1507

NCT ID:

NCT00897143

Start Date:

June 2007

Completion Date:

Related Keywords:

  • Lung Cancer
  • Metastatic Cancer
  • non-small cell lung cancer
  • malignant pleural effusion
  • small cell lung cancer
  • Lung Neoplasms
  • Neoplasm Metastasis
  • Neoplasms
  • Neoplasms, Second Primary
  • Pleural Effusion
  • Pleural Effusion, Malignant

Name

Location

Case Medical Center, University Hospitals Seidman Cancer Center, Case Comprehensive Cancer Center Cleveland, Ohio  44106-5065