Isolation and Identification of Lung Cancer Precursor Cells From Malignant Pleural Effusion Specimens
OBJECTIVES:
- Isolate and identify lung cancer precursor cells in discarded pleural fluid specimens
obtained during routine thoracentesis procedures for the diagnosis and treatment of
malignant pleural effusions.
OUTLINE: Malignant pleural effusion specimens obtained from the Pathology Department at the
University Hospitals of Cleveland are analyzed using an immunomagnetic bead enrichment assay
to isolate cells of epithelial origin. The isolated cells are then stained with fluorescent
DNA binding dye, Hoechst 33342, and other markers (i.e., CD133, CD24/CD44) and sorted by
flow cytometry into progenitor and nonprogenitor subpopulations. The sorted subpopulations
are separated and examined functionally by in vitro and in vivo studies. Sphere culture
studies are also performed on the sorted subpopulations. The specimens may also be utilized
for transcriptional profiling studies, RNAi studies, and for the establishment of long-term
cultures and cell lines. DNA is also isolated and stored from tumor and nonepithelial normal
cell compartments for future genetic studies on known and unidentified genetic abnormalities
contributing to the development of lung cancer, such as EGFR, ErbB2, K-ras, and p53
mutational status.
Observational
Time Perspective: Retrospective
Isolation and identification of lung cancer precursor cells in malignant pleural effusion specimens
No
Balazs Halmos, MD
Principal Investigator
Case Medical Center, University Hospitals Seidman Cancer Center, Case Comprehensive Cancer Center
United States: Institutional Review Board
CASE1507
NCT00897143
June 2007
Name | Location |
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Case Medical Center, University Hospitals Seidman Cancer Center, Case Comprehensive Cancer Center | Cleveland, Ohio 44106-5065 |