Know Cancer

or
forgot password

CMV Specific Cellular Immunity in Recipients of Allogeneic Bone Marrow Transplantation: Association of CMV-Specific HLA-Peptide Tetramer Binding With Cytotoxic T-Cell Function, CMV Infection and Other Clinical Events


N/A
18 Years
N/A
Not Enrolling
Both
Chronic Myeloproliferative Disorders, Leukemia, Lymphoma, Multiple Myeloma and Plasma Cell Neoplasm, Myelodysplastic Syndromes, Myelodysplastic-Myeloproliferative Diseases, Nonneoplastic Condition

Thank you

Trial Information

CMV Specific Cellular Immunity in Recipients of Allogeneic Bone Marrow Transplantation: Association of CMV-Specific HLA-Peptide Tetramer Binding With Cytotoxic T-Cell Function, CMV Infection and Other Clinical Events


OBJECTIVES:

- To document the quantitative characteristics of a cytomegalovirus (CMV)-specific
HLA-peptide tetramer-binding assay (TBA) for cytotoxic T lymphocytes (CTLs) in patients
who have undergone allogeneic bone marrow transplantation (BMT) or peripheral blood
stem cell transplantation (PBSCT).

- To confirm the optimal TBA conditions for CTL characterization in these patients.

- To compare the TBA results for these patients with conventional assays for CTL
functions.

- To compare CMV-specific TBA during the first 3 months after allogeneic BMT or PBSCT and
to determine whether this binding function, in either donor or recipient, is a
surrogate marker for protection from risk of CMV infection in these patients.

- To determine whether acquisition of CMV-specific TBA protects from risk for CMV disease
in patients having active CMV infection after allogeneic BMT or PBSCT.

OUTLINE: This is a multicenter study. Patients accrue initially to cohort 1 until a
sufficient number of stem cell transplantation (SCT) recipients are enrolled. Patients then
accrue to cohort 2 based on documented cytomegalovirus (CMV) infection and preemptive
treatment with ganciclovir within 90 days after SCT (patients previously accrued to cohort 1
can be accrued to cohort 2 if they develop CMV infection).

- Cohort 1: Patients undergo blood sample collection on approximately days 40, 90, 120,
150, 180, and 360 post transplantation. Samples are analyzed (to determine the
development of CMV immunity) for prevalence of CMV-specific cytotoxic T-lymphocytes
(CTL) by HLA-peptide tetramer-binding assay (TBA) pp65, with and without in vitro
stimulation (IVS). Samples collected on days 40 and 90 are also analyzed by chromium
release assay (CRA). Patients suspected of developing CMV viremia may receive
ganciclovir according to standard clinical practice.

- Cohort 2: Patients undergo blood sample collection on approximately days 90, 120, 150,
180, and 360 after SCT. Samples are analyzed by TBApp65 staining and for prospective
measurement of CMV-specific CTL functions.

All patients undergo routine clinical surveillance for CMV infection on days 21 to 100 after
SCT. CMV viral load measurements are obtained twice weekly by CMV-DNA PCR assays on blood
cells and plasma and shell-vial blood cultures. In cohort 2, CMV viral load is also
determined on days 90 (if not previously as part of cohort 1), 120, 150, 180, and 360. The
CMV infection data obtained is then compared with TBA and CTL measurements using
HLA-specific tetramers and IVS-induced cytotoxicity assays. Clinical events, such as
graft-versus-host disease, underlying disease status, and procedure-related complications
are also analyzed and correlated with TBA results.

Stromal cell cultures are obtained from marrow donors at the time of marrow harvest for use
as target cells in CTL assays. Donor saliva samples are also obtained for detection of CMV
infection by shell-vial method.

Inclusion Criteria


DISEASE CHARACTERISTICS:

- Meets 1 of the following criteria at the City of Hope National Medical Center:

- Patient who has undergone a matched-related or matched-unrelated allogeneic bone
marrow or peripheral blood stem cell transplantation (SCT) for a hematological
malignancy (e.g., aplastic anemia or myelodysplastic syndromes)

- At risk for cytomegalovirus (CMV) infection and disease due to 1 of the
following risk factors:

- CMV-seropositive prior to transplantation

- Received SCT from a CMV-seropositive donor

- Donor for matched-related SCT

- Healthy volunteer evaluated concurrently with SCT recipients to establish normal
values for both CMV-seronegative and CMV-seropositive persons

- Any HLA serotypes allowed

PATIENT CHARACTERISTICS:

- See Disease Characteristics

PRIOR CONCURRENT THERAPY:

- See Disease Characteristics

Type of Study:

Interventional

Study Design:

Primary Purpose: Supportive Care

Outcome Measure:

Quantitative determination of HLA-peptide tetramer-binding assay (TBA) pp65 on days 40 and 90 after stem cell transplantation, with and without in vitro stimulation

Safety Issue:

No

Principal Investigator

John A. Zaia, MD

Investigator Role:

Principal Investigator

Investigator Affiliation:

Beckman Research Institute

Authority:

United States: Federal Government

Study ID:

99061

NCT ID:

NCT00716911

Start Date:

January 2000

Completion Date:

Related Keywords:

  • Chronic Myeloproliferative Disorders
  • Leukemia
  • Lymphoma
  • Multiple Myeloma and Plasma Cell Neoplasm
  • Myelodysplastic Syndromes
  • Myelodysplastic-Myeloproliferative Diseases
  • Nonneoplastic Condition
  • cytomegalovirus infection
  • accelerated phase chronic myelogenous leukemia
  • atypical chronic myeloid leukemia, BCR-ABL negative
  • blastic phase chronic myelogenous leukemia
  • chronic myelomonocytic leukemia
  • chronic phase chronic myelogenous leukemia
  • recurrent adult acute lymphoblastic leukemia
  • recurrent adult acute myeloid leukemia
  • refractory chronic lymphocytic leukemia
  • refractory hairy cell leukemia
  • relapsing chronic myelogenous leukemia
  • secondary acute myeloid leukemia
  • stage III chronic lymphocytic leukemia
  • stage IV chronic lymphocytic leukemia
  • splenic marginal zone lymphoma
  • extranodal marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue
  • nodal marginal zone B-cell lymphoma
  • stage III adult Burkitt lymphoma
  • stage III adult diffuse large cell lymphoma
  • stage III adult diffuse mixed cell lymphoma
  • stage III adult diffuse small cleaved cell lymphoma
  • stage III adult immunoblastic large cell lymphoma
  • stage III adult lymphoblastic lymphoma
  • stage III grade 1 follicular lymphoma
  • stage III grade 2 follicular lymphoma
  • stage III grade 3 follicular lymphoma
  • stage III mantle cell lymphoma
  • stage III marginal zone lymphoma
  • stage III small lymphocytic lymphoma
  • stage IV adult Burkitt lymphoma
  • stage IV adult diffuse large cell lymphoma
  • stage IV adult diffuse mixed cell lymphoma
  • stage IV adult diffuse small cleaved cell lymphoma
  • stage IV adult immunoblastic large cell lymphoma
  • stage IV adult lymphoblastic lymphoma
  • stage IV grade 1 follicular lymphoma
  • stage IV grade 2 follicular lymphoma
  • stage IV grade 3 follicular lymphoma
  • stage IV mantle cell lymphoma
  • stage IV marginal zone lymphoma
  • stage IV small lymphocytic lymphoma
  • recurrent adult Hodgkin lymphoma
  • recurrent adult Burkitt lymphoma
  • recurrent adult diffuse large cell lymphoma
  • recurrent adult diffuse mixed cell lymphoma
  • recurrent adult diffuse small cleaved cell lymphoma
  • recurrent adult immunoblastic large cell lymphoma
  • recurrent adult lymphoblastic lymphoma
  • recurrent cutaneous T-cell non-Hodgkin lymphoma
  • recurrent mycosis fungoides/Sezary syndrome
  • recurrent grade 1 follicular lymphoma
  • recurrent grade 2 follicular lymphoma
  • recurrent grade 3 follicular lymphoma
  • recurrent mantle cell lymphoma
  • recurrent marginal zone lymphoma
  • recurrent small lymphocytic lymphoma
  • chronic eosinophilic leukemia
  • chronic neutrophilic leukemia
  • primary myelofibrosis
  • stage II multiple myeloma
  • stage III multiple myeloma
  • refractory multiple myeloma
  • de novo myelodysplastic syndromes
  • previously treated myelodysplastic syndromes
  • secondary myelodysplastic syndromes
  • myelodysplastic/myeloproliferative neoplasm, unclassifiable
  • stage I multiple myeloma
  • Neoplasms
  • Cytomegalovirus Infections
  • Leukemia
  • Lymphoma
  • Lymphoma, Non-Hodgkin
  • Multiple Myeloma
  • Neoplasms, Plasma Cell
  • Plasmacytoma
  • Myelodysplastic Syndromes
  • Preleukemia
  • Myeloproliferative Disorders
  • Lymphoma, Large-Cell, Immunoblastic
  • Myelodysplastic-Myeloproliferative Diseases

Name

Location