Inclusion Criteria:

DISEASE CHARACTERISTICS

1. advanced bladder cancer which already showed resistance to standard treatments

2. Protein expression of MPHOSPH1 and DEPDC1 on the tumor

PATIENTS CHARACTERISTICS

1. Patients who showed resistance to standard chemotherapies or radiotherapy

2. Histological diagnosis is transitional cell carcinoma

3. HLA-A*2402

4. ECOG performance status of 0 to 1

5. Age ≥ 20 years, ≤80 years

6. WBC≥ 2,000/mm³, ≤15000/mm³ Platelet count ≥ 75000/mm³ AST, ALT ≤150
IU/l Total bilirubin ≤ 3.0 mg/dl Creatinine ≤ 3.0 mg/dl

7. lesion of bladder cancer must express MPHOSPH1 or DEPDC1

8. Able and willing to give valid written informed consent

Exclusion Criteria:

1. Pregnancy (women of childbearing potential: Refusal or inability to use effective
means of contraception)

2. Breastfeeding

3. Patients willing to childbearing ( Refusal or inability to use effective means of
contraception)

4. Serious infections requiring antibiotics

5. Concomitant treatment with steroids or immunosuppressing agent

6. Other malignancy difficult to control.

7. Decision of unsuitableness by principal investigator or physician-in-charge