Inclusion Criteria:

1. Healthy males and female subjects

2. Between the ages of 18 to 45

3. BMI between 18.5 kg/m2 to 24. 9 kg/m2 (normal weight) and between 28.0 kg/m2 to 39.9
kg/m2 (overweight)

4. Willingness to refrain from alcohol during the study

5. Able to come to the FHCRC in Seattle every weekday night for dinner

Exclusion Criteria:

1. Younger than 18 years of age or older than 45 years of age.

2. Do not fit into one of the study weight groups (normal weight and overweight): BMI <
18.5 kg/m2 or > 40.0 kg/m2, and between 25.0 kg/m2and 27.9 kg/m2

3. Have diseases that are treated by diet and/or medications including but not limited
to diabetes, kidney disease, heart disease

4. Taking prescription medications every day (this includes women taking birth-control
pills, shots, patch or IUD with hormones)

5. Diagnosed with or treated for cancer within the previous five years (except those
with a diagnosis and/or treatment of non-melanomatous skin cancer are eligible)

6. Currently pregnant or breastfeeding or planning a pregnancy in the next 3 months.

7. Using any tobacco products on a daily basis (cigarettes, pipes, cigars, chewing
tobacco).

8. Using recreational drugs

9. Drinking the following amount of alcohol almost every day: 2 or more cans/bottles of
beer OR 2 or more glasses of wine OR 3 or more ounces of hard liquor.

10. Inability (e.g., food allergy or intolerances) or unwillingness to consume the foods
that are part of the feeding study diet.