Phase I Trial to Assess Safety and Immunogenicity of Xenogeneic CD20 DNA Vaccination With Patients With B-Cell Lymphoma
- Patients must have a functional immune system as determined by the following tests:
- Serum proteins immunoelectrophoresis (serum IgG levels ≥ 0.5 g/dL are required)
- No evidence of anergy as shown by positive skin test with tetanus toxoid, mumps or
- Circulating T-cells as measured by flow cytometry (serum CD4+ and CD8+ T-cell counts
≥ 250 and 150 cells/μL, respectively) Patients must have histologically proven (and
confirmed at MSKCC) B-cell lymphoma of any histology, excluding Burkitt's lymphoma,
Lymphoblastic lymphoma (due to their aggressiveness and low likelihood of response to
- CD20 surface expression must be confirmed by immunohistochemical staining or flow.
- Measurable disease is not a pre-requisite for enrollment in the study. However, if a
patient does have measurable disease as evidenced by imaging studies, these have to
be done within eight weeks of starting treatment.
- Patients must have a Karnofsky performance status ≥ 70%.
- Patients with evidence of active disease, progression of disease or relapsed disease
following one or more prior regimens of chemotherapy, immunotherapy or radiation
therapy (including autologous stem cell transplants), not requiring immediate
cytoreductive chemotherapy. All treatment must be completed at least four weeks prior
to administration of the first vaccination, except immunotherapy and
radioimmunotherapy, which must be completed at least 90 days prior to receiving the
first vaccination. Active disease includes patients with minor or partial responses
after therapy as evidenced by FDG-avid disease or biopsy.
- Age ≥ 18.
- Adequate contraception during study enrollment.
- Avoidance of breast-feeding their infants during the study enrollment.
- Patients must have adequate organ and marrow function as defined below:
- Absolute Neutrophil Count ≥ 1,000/uL
- Platelets ≥ 75,000/uL
- Total bilirubin ≤ 2.5 times institutional upper limit
- AST/ALT ≤ 2.5 times institutional upper limit
- Creatinine ≤ 2 mg/dL
- PT/PTT ≤ 1.5 times institutional upper limit
- Patients must have no signs of congestive heart failure according to the New York
Heart Failure Guidelines Class III/IV.
- Patients who have had chemotherapy or radiation therapy within 4 weeks prior to
entering the study.
- Patients who have undergone an allogeneic stem cell transplant at any time.
- Patients who have not recovered from adverse events due to agents administered more
than 4 weeks earlier.
- Patients who have received immunotherapy (i.e. rituximab) or radioimmuno therapy
(i.e. tositumomab or ibritumomab) within the past 90 days.
Patients who display signs of anergy as indicated by skin testing.
- Patients with Burkitt's lymphoma and Lymphoblastic Lymphoma.
- Patients who have been previously immunized with any type of DNA vaccine.
- Patients who have positive anti-DS-DNA antibodies.
- Patients with life expectancy less than 3 months from the time of enrollment.
- Patients with serious underlying medical conditions, active infections requiring the
use of antimicrobial drugs or active bleeding.
- Patients with active Hepatitis C (HC) or Hepatitis B (HB) infection, the latter
defined as a positive test for HBsAg or measurable viral load. In patients who are
HBsAg negative but HBsAg positive (regardless of HBsAb status), a HB viral load will
be performed and if positive the subject will be excluded. If the subject is HBsAg
negative, HBcAb positive (regardless of HBsAb status) but with negative HBV viral
load, the subject may be included but must undergo HBV DNA PCR testing at least every
two months from the start of treatment during the routine study visits for as long as
the subject remains on study. Prophylactic antiviral therapy, in addition to the
monitoring described above, may be initiated at the discretion of the investigator.
Patients with documented HIV infection or other immunodeficiency disorder or on chronic
- Patients with autoimmune diseases such as but not limited to rheumatoid arthritis,
Sjogren disease, ulcerative colitis, autoimmune hepatitis.
- Pregnant or nursing women. Women of child-bearing age will be tested for qualitative
β-HCG within 2 weeks of immunization.
- Patients receiving other investigational drug.