Trial Information

Role of Cytochrome P450 2D6 (CYP2D6) *4 Polymorphism, Oxidative Stress and Ferretin in Benign and Malignant Breast Diseases

Breast cancer is the most common malignancy among women and the second highest cause of
cancer death. Nevertheless most of the breast cancer is sporadic with no family history of
the disease, Enhanced CYP2D6 activity has been related to malignancies of the bladder,
liver, pharynx, and stomach and, especially, to cigarette-smoking-induced lung cancer. Data
support that increased formation of reactive oxygen species (ROS) may play an important role
in carcinogenesis Numerous in vitro experiments show that ROS damages DNA, inducing
premutagenic modifications of nucleotides and promoting oxidation of proteins and lipid per
oxidation The aim of our study is to investigate the potential role of the CYP2D6*4
polymorphism in breast cancer and furthermore to assess the role of oxidative stress,
antioxidant capacity and ferretin in pathogenesis of breast cancer in different CYP2D6*4
genotypes.patients and methods : This study included 31 women admitted to the general
surgery department, Mansoura University Hospital. group I consists of 15 women with benign
breast lesion, group II composed of 16 women with cancer breast and 13 healthy women with
matched age and clinically free were taken as a control group. DNA extraction and genotyping
of cytochrome P450-2D6*4 gene using conventional PCR followed by restriction enzyme (Mva1)
digestion. Separated plasma was used for measurement of plasma
malondialdehyde(MDA)concentration, total plasma antioxidant capacity and plasma ferretin
level.Results homozygous genotype of 2D6*4 represent 56.25 %( 9/16) of breast cancer group
with lower wild type (31.25%-5/16), while it represent 33.3 %( 5/15) of begnine breast
lesion group and 15.3(2/13) of the control group. Where the wild type represent the majority
of cases (61.15%-8/13) in the control group and 46.6%-7/15) in the begnine breast lesion
group.

statistically significant increase in plasma ferretin and MDA mean levels in breast cancer
group than begnine breast lesion and the control groups .Also, there is an increase in
plasma total antioxidant activity in breast cancer group than the control group with
nonstatistical difference between the breast cancer and the benign breast lesion groups .

Conclusion CYP2D6*4 genotype polymorphism interacting with plasma malondialdehyde (MDA)
oxidative stress and plasma ferretin level may have a role in the pathogenesis of breast
cancer