Genetic Factors Affecting Breast Cancer Progression
- Correlate inherited and acquired variations in candidate genes with breast cancer
progression and survival in women with newly diagnosed or metastatic breast cancer.
- Establish a repository of blood and tumor samples from these patients, linked to a
database of clinical, pathological, and treatment outcome data for future research
OUTLINE: Patients complete epidemiological questionnaires and undergo blood sample
collection. Tumor tissue specimens are requested from the pathology departments.
Blood samples are analyzed for single nucleotide polymorphisms and other polymorphic
variants in candidate genes by polymerase chain reaction. Candidate genes include genes
involved in the DNA damage response, programed cell death, inflammation, and angiogenesis.
Tumor samples are analyzed by tissue microarrays using immunohistochemistry, denaturing
high-performance liquid chromatography, and DNA sequencing to study genetic changes in the
PROJECTED ACCRUAL: A total of 1,800 patients will be accrued for this study.
Correlation of inherited and acquired variations in candidate genes with breast cancer progression and survival
Angela Cox, PhD
University of Sheffield
United States: Federal Government