Prevention of Thiopurines Related Toxicity Through the Determination by DHPLC TPMT Polymorphisms in Patients With ALL.
Objectives: Determine by DHPLC analysis the frequency of TPMT polymorphisms in mestizos
mexican population with acute lymphoblastic leukemia.
- To do a clinical correlation between the presence of polymorphism and thiopurine
- Inclusion criteria: Healthy volunteers or patients with acute lymphoblastic leukemia,
age > 18 years, who attend to the National Institute of Cancerologia.
- Exclussion criteria: Patients with ALL, who are unable to have an adequate follow-up.
- Samples: Genomic DNA from peripheral blood leukocytes was isolated by standard methods.
Known (wild-type and polymorphic) sequenced polymerase chain reaction (PCR) fragments
of the TPMT gene were used as controls. TPMT gene fragments were amplified. PCR
products were then analyzed by denaturating high performance liquid chromatography
Additional Descriptors: Convenience Sample, Primary Purpose: Screening, Time Perspective: Cross-Sectional, Time Perspective: Prospective
Myrna Candelaria, MD
National Institute of Cancerologia
Mexico: Ethics Committee