Prevention of Thiopurines Related Toxicity Through the Determination by DHPLC TPMT Polymorphisms in Patients With ALL.
18 Years
N/A
Both
Acute Lymphoblastic Leukemia
Trial Information
Prevention of Thiopurines Related Toxicity Through the Determination by DHPLC TPMT Polymorphisms in Patients With ALL.
Objectives: Determine by DHPLC analysis the frequency of TPMT polymorphisms in mestizos
mexican population with acute lymphoblastic leukemia.
- To do a clinical correlation between the presence of polymorphism and thiopurine
related- myelotoxicity.
- Inclusion criteria: Healthy volunteers or patients with acute lymphoblastic leukemia,
age > 18 years, who attend to the National Institute of Cancerologia.
- Exclussion criteria: Patients with ALL, who are unable to have an adequate follow-up.
- Samples: Genomic DNA from peripheral blood leukocytes was isolated by standard methods.
Known (wild-type and polymorphic) sequenced polymerase chain reaction (PCR) fragments
of the TPMT gene were used as controls. TPMT gene fragments were amplified. PCR
products were then analyzed by denaturating high performance liquid chromatography
(DHPLC).
Inclusion Criteria:
- Healthy and patientes with acute lymphoblastic leukemia.
- Age: older than 18 years.
- Attend to the National Institute of Cancerologia
Exclusion Criteria:
- Foreign patients with an irregular attendance to the National Institute of
Cancerologia
Type of Study:
Observational
Study Design:
Additional Descriptors: Convenience Sample, Primary Purpose: Screening, Time Perspective: Cross-Sectional, Time Perspective: Prospective
Principal Investigator
Myrna Candelaria, MD
Investigator Role:
Principal Investigator
Investigator Affiliation:
National Institute of Cancerologia
Authority:
Mexico: Ethics Committee
Study ID:
005/007/ICI
NCT ID:
NCT00402090
Start Date:
April 2005
Completion Date:
November 2006
Related Keywords:
- Acute Lymphoblastic Leukemia
- thiopurines
- thiopurine methyl transferase
- toxicity
- pharmacogenomics
- Leukemia
- Leukemia, Lymphoid
- Precursor Cell Lymphoblastic Leukemia-Lymphoma
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