Inclusion Criteria:

A) Age 18 years or older B) Patients with confirmed or suspected non-small cell lung
cancer who require a mediastinoscopy as part of their staging investigations of the
mediastinum to determine suitability for lung cancer resection will be considered for the
trial.

C) Patients with undiagnosed enlarged lymph nodes in the mediastinum suspicious for lung
cancer in which a tissue diagnosis is required.

Exclusion Criteria:

A) Patients who are deemed on clinical grounds not to be medically fit for a bronchoscopy
or a mediastinoscopy or who are not suitable for definitive surgical resection by
thoracotomy will be excluded.

B) Patients who have verified stage IV disease or who are not appropriate for lung cancer
resection by virtue of direct invasion of mediastinal structures or large parts of the
chest wall.

C) Known small cell lung cancer. D) Patients where there is a high clinical suspicion of
lymphoma. E) Inability to give informed consent.