T Cell Repertoire Analysis of Immune Mediated Skin Diseases
The aim of the study is to characterized the T cell repertoire of individuals with immune
mediated skin disease (e.g. psoriasis and mycosis fungoides). Peripheral blood with be
collected from volunteers with psoriasis, mycosis fungoides and age matched controls.
Fifteen tablespoons of blood will be collected prior to the initiation of treatment and
again after the patient shows a clinical response to treatment. The time between blood
draws will be no less than 3 months. There will be no more than two blood draws per
patient. Blood samples will be used to determine the patient's HLA haplotype via PCR and
DNA sequencing. After the patient's haplotype has been established the activated T cell
repertoire will be analyzed for clonal expansions. Clonal expansions in the T cell
repertoire will be determined by immunoscope analysis, which is a PCR based technique.
Observational Model: Case Control, Time Perspective: Prospective
Peripheral blood will be collected from adults ages 18-55. These samples will then be used for PCR analysis and T cell cloning.
Emanual Maverakis, MD
University of California, Davis
United States: Institutional Review Board
|University of California, Davis Department of Dermatology||Sacramento, California 95816|