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Influence of Cholestasis on Intestinal BCRP Expression

18 Years
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Trial Information

Influence of Cholestasis on Intestinal BCRP Expression

BCRP is capable of transport bile acids and may indicate that this transporter is involved
in bile acid homeostasis. As an efflux pump, it could protect the enterocytes from potential
toxic bile acid concentrations. During cholestasis, where the enterohepatic circulation is
disrupted, there occurs an adaptive regulation of transporters for bile acids, bilirubin and
cholesterol. These changes take place in liver, kidney, as well as in the intestine.

Using real-time RT-PCR analysis we determine BCRP mRNA expression levels in duodenal tissue
of healthy subjects and cholestatic patients. BCRP protein levels will be determined by

Healthy subjects and cholestatic patients are enrolled in the study after giving informed
consent. Control subjects with an indication for a gastrointestinal tract endoscopy within a
cancer-screening program and patients with obstructive cholestasis with an interventional
endoscopic retrograde cholangiopancreatography (ERCP) are included in this study. During
endoscopy four biopsy specimens are obtained from the distal part of the duodenum. Biopsies
are immediately stored at –70°C until further processing.

Inclusion Criteria:

- clinical diagnosis of obstructive cholestasis (obstructive jaundice was defined on
the basis of chemical parameters (bilirubin, g-glutamyltransferase, and alkaline
phosphatase) and on imaging procedures (ultrasound and ERCP) demonstrating a dilated
bile duct system)

- control subjects had an indication for a gastrointestinal tract endoscopy within a
cancer-screening program

Exclusion Criteria:


Type of Study:


Study Design:

Observational Model: Defined Population, Observational Model: Natural History, Time Perspective: Cross-Sectional, Time Perspective: Prospective

Principal Investigator

Jürgen Drewe, MD, MSc

Investigator Role:

Principal Investigator

Investigator Affiliation:

Department of Clinical Pharmacology, University Basel


Switzerland: Ethikkommission

Study ID:




Start Date:

June 2003

Completion Date:

August 2004

Related Keywords:

  • Icterus
  • cholestasis
  • BCRP
  • multidrug resistance
  • duodenum
  • bile acids
  • ABCG2
  • Cholestasis
  • Jaundice