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In Vitro Evaluation of Immune Responses in CTCL

18 Years
Not Enrolling
Cutaneous T-Cell Lymphoma, Sezary Syndrome, Mycosis Fungoides

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Trial Information

In Vitro Evaluation of Immune Responses in CTCL

This is an in vitro evaluation of cutaneous T-cell lymphoma using patients blood and tissue
to evaluate immune responses related to identified tumor populations and dendritic/ CD 8
cells with the following objectives:

- Objective I: Evaluate the feasibility of the preparation of dendritic cells (DCs), CD8
and Sezary cells (all CD4 positive) in vitro, obtained from the buffy coats or a skin
biopsy from an area with tumor involvement of subjects with cutaneous t-cell lymphoma
(CTCL) and Sezary syndrome (leukemic stage of CTCL) undergoing an approved FDA therapy,
extracorporeal photopheresis (ECP).

- Objective II: Evaluate antigen loading of the DCs.

- Objective III: Test the functional capacities of the DCs derived from CTCL subjects to
overcome immune tolerance to the tumor cells ex-vivo, by using modified Elispot assay
in conjunction with Granzyme B Elispot assay as a measurement of cytotoxicity.

Inclusion Criteria:

- Males or females <18 years of age

- Histologically confirmed stage IV cutaneous T-cell lymphoma, with at least 5% of the
peripheral blood lymphocytes showing atypical morphology consistent with Sezary cells

- Ambulatory and be in stable medical condition

- Biopsy positive mycosis fungoides/CTCL or clonal type of CTCL as determined by PCR
for TCR and Southern blot for TCR

Exclusion Criteria:

- Received any chemotherapy or radiotherapy within 4 weeks prior to enrollment

- Significant psychiatric illness which would prevent adequate informed consent in the
opinion of the principal investigator

- Systemic steroid therapy other than maintenance for adrenal suppression

- Known coagulopathy for non SS subjects.

Type of Study:


Study Design:

Time Perspective: Prospective

Principal Investigator

Larisa J. Geskin, M.D.

Investigator Role:

Principal Investigator

Investigator Affiliation:

University of Pittsburgh


United States: Institutional Review Board

Study ID:




Start Date:

June 2002

Completion Date:

January 2006

Related Keywords:

  • Cutaneous T-cell Lymphoma
  • Sezary Syndrome
  • Mycosis Fungoides
  • Cutaneous T-cell Lymphoma
  • Sezary Syndrome
  • Mycosis Fungoides
  • Immune Responses
  • Lymphoma
  • Mycoses
  • Mycosis Fungoides
  • Sezary Syndrome
  • Lymphoma, T-Cell
  • Lymphoma, T-Cell, Cutaneous



University of Pittsburgh Medical Center Pittsburgh, Pennsylvania  15213