Correlation of PTEN and IGFBP-3 in the Invasion of Ovarian Endometrioid Carcinoma
We have successfully established an ovarian cancer cell line (OVTW-59), which was derived
from an ovarian endometrioid carcinoma (EC), and have also established an ovarian EC
invasion model. By using cDNA microarray and quantitative reverse-transcriptase polymerase
chain reaction, we identified insulin-like growth factor binding protein (IGFBP)-3 as an
invasion suppressor gene, which were associated with lower cancer migration, invasion and
metastasis. Clinically, lower IGFBP-3 was found associated with significantly higher tumor
grade, advanced stage and poor survival in patients with EC tumors. Furthermore, we have
proved IGFBP-3 expression correlated with lower Erk activation, but with no effect on the
activation of Akt. All these two signal transduction proteins have crucial roles in cancer
invasion. Recently, a novel model of ovarian EC formation from endometriosis was reported,
and PTEN was found to be a major protein involved. Inactivation of PTEN has been reported in
some ovarian EC tumors and methylation was suggested as one of the major epigenetic changes.
This tumorigenesis model has lots of similarity to our established invasion model.
Therefore, we plan to study the important of PTEN expression in ovarian EC and if
inactivation of PTEN and IFGBP-3 is through methylation. Furthermore, by studying the signal
transduction pathways using PTEN and IGFBP-3 transfection, we plan to study the mutual
interaction between PTEN and IGFBP-3 on the suppression of tumor invasion in ovarian EC.
Endpoint Classification: Bio-equivalence Study, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Diagnostic
Torng Pao-Ling, MD, PhD
Department of Obstetric and Gynecology, National Taiwan University Hospital
Taiwan: Department of Health