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An Open Label, Multicenter, Non Randomized Phase II Study to Evaluate Anti-Tumor Activity and Safety of a Combination of Fludarabine, Mitoxantrone and Rituximab in Relapsed or Primary Failing Advanced Follicular Non-Hodgkin's Lymphoma.

Phase 2
18 Years
75 Years
Not Enrolling
Follicular Lymphoma

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Trial Information

An Open Label, Multicenter, Non Randomized Phase II Study to Evaluate Anti-Tumor Activity and Safety of a Combination of Fludarabine, Mitoxantrone and Rituximab in Relapsed or Primary Failing Advanced Follicular Non-Hodgkin's Lymphoma.

Follicular non Hodgkin's lymphoma's (FL), as defined by the REAL Classification, are usually
characterized by a slowly progressive clinical course, a transient control by standard
chemotherapeutic regimen and a pattern of repeated relapses until ultimately progressive and
fatal disease.

Standard first line treatment for advanced FL consists of alkylating-based (CVP) or
anthracycline containing regimen in association to interferon alpha (CHVP+IFN) chemotherapy.
Others approaches have been developed mostly as secondary therapy including purine analogs
alone or in combination with alkylators or mitoxantrone, high dose therapy with autologous
peripheral stem cell transplantation and, more recently, treatment with the unconjugated
chimeric anti-CD20 antibody (rituximab) to target the CD20 antigen highly expressed on
follicular lymphoma cells. None of these strategies does appear to give a definitive
survival advantage. Thus, in patients with FL, the design of novel combination programs is a
major challenge.

Combination of fludarabine and mitoxantrone in low grade, predominantly Follicular NHL:
results of phase II studies in relapsed or refractory patients Fludarabine is expected to
potentiate other agents through inhibition of DNA polymerase alpha and DNA ligase and its
consequent interference with the DNA repair process. The addition of mitoxantrone increases
the cytotoxic effect of fludarabine in vitro. McLaughlin et al developed a combination of
fludarabine, mitoxantrone and dexamethasone (FND), which was very effective in 51 patients
with recurrent low-grade lymphoma (including 65% FL), with an overall response rate of 94%
(47% complete response (CR) rate. The median duration of response in this phase II study was
21 months for CR patients but only 9 months for partial responders (PR) patients. The median
survival and failure-free survival times from the time of entry onto the FND study were 34
and 14 months, respectively. Most major responses were evident after two to four courses of
chemotherapy. The need for continuation of therapy beyond attainment of remission is
suggested by early relapses among patients who had early discontinuation of therapy. The
predominant toxic effects were myelosuppression and infections: neutropenia < 500/µl in 20 %
of courses, thrombopenia < 50000/µl in 8 % of courses and infections in 12 % of courses.
Non-hematological toxicity was modest.

FND appears to be comparable to, and less toxic than the combination of etoposide,
methylprednisolone, cytarabine, and cisplatin (ESHAP), one of the most effective regimens
available for patients with relapsed indolent lymphoma. Others studies have confirmed the
significant efficacy and moderate toxicity profile of this combination as salvage therapy in
low grade, predominantly follicular lymphoma.

Moreover, the omission of corticosteroids reduces the risk of opportunistic infections,
while the activity of the combination against indolent lymphoma is maintained.

Preliminary data from rituximab studies alone or in combination with chemotherapy in
relapsed or refractory low grade LNH In vitro, rituximab mediates complement dependent
cytotoxicity (CDC), antibody dependent cellular cytotoxicity (ADCC) and apoptosis. However,
the mechanism of in vivo anti-lymphoma effect remains largely unknown. Rituximab received
approval for recurrent follicular lymphoma based on response rates of about 50% including 6%
complete responses and duration of responses, which compare favorably to that of all other
single agents including fludarabine and 2-CdA (15-19). Median time to progression for
responders is around 13 months. Toxicity of rituximab is low and easily manageable. An 8
doses schedule did not show to confer a significant advantage in term of response rate and
duration of response over the four doses schedule.

Rituximab has been shown to sensitize drug-resistant lymphoma cell lines to killing by
cytotoxic drugs including fludarabine.

Thus, we may hypothesize that the combination of rituximab, fludarabine and mitoxantrone
might lead to synergistic / additive induction of apoptosis through different pathways in
lymphoma B-cells which maintain an indolent growth pattern.

This approach may provide a means to achieve longer progression free survival in relapsed or
refractory patients with FL.

We opted for a four induction cycles of rituximab, fludarabine and mitoxantrone since:

1. Four cycles of a combination of fludarabine and mitoxantrone are generally sufficient
to assess response,

2. the 4 doses schedule of rituximab which has been the most studied is efficient 3) The
omission of dexamethasone does not appear to impair ORR and DR of a combination of
fludarabine and novantrone . Recycling will start on day 28.

Subsequently responding patients according the International criteria Working group will
have 2 more cycle of a combination of fludarabine and mitoxantrone but no rituximab.

Inclusion Criteria:

- 18 years < age < 75 years

- Pathologically confirmed low grade, follicular, B cell lymphoma (WHO Classification
Follicular grades 1 and 2

- Failed at least first line chemotherapy with any standard anthracycline containing
regimen (see appendix C for definition of treatment failure)

- Frozen biopsy material obtained at relapse or disease progression should be available
for central pathology review and molecular biology studies

- The lymphoma must be CD20 positive (on the biopsy material obtained at relapse or
disease progression)

- At least one measurable lesion one nodal or extranodal lesion

- WHO performance status grade 0 or 1

- Bulky disease at study entry according to the GELF criteria: Nodal or extranodal
single mass > 7cm in its greatest diameter; systemic B-symptoms; increased LDH and
beta 2 microglobulinemia (> 3mg/L); involvement of at least 3 nodal sites, each with
a diameter of greater than 3 cm; splenic enlargement with margin below the umbilical
line or cranio caudal diameter of greater than 20 cm; compression syndrome (ureteral,
orbital, gastrointestinal), or pleural or peritoneal serous effusion.

- Patient information and written informed consent

Exclusion Criteria:

- Evidence of histological transformation to diffuse large B-cell lymphoma

- > 2 prior treatment regimen

- Chemotherapy, or other experimental anticancer treatment during the 4 weeks before

- Any radiation therapy to the index lesion(s) during the 4 weeks before inclusion

- Autologous stem cell transplant during the 3 months before inclusion

- Prior treatment including fludarabine and / or mitoxantrone and / or rituximab or
contra-indication to one of these products

- Unless exempted by the Responsible Investigator, as lymphoma related: serum
creatinine >2 x Institutional Upper Limit of Normal (IULN), total bilirubin >2 x IULN
or AST (SGOT) >2 x IULN, alkaline phosphatase >2 x IULN

- Low bone marrow function: absolute neutrophil count < 1500/mm3 and platelet < 100 x
109/L at study entry (unless bone marrow infiltration)

- Clinically significant cardiac disease, as defined by history of symptomatic
ventricular arrhythmias, congestive heart failure or myocardial infarction within 12
months of study entry

- Evidence of symptomatic CNS disease

- Known positivity for HIV, HBs antigen or hepatitis C

- Pregnant or lactating women. Women of childbearing potential, and all men, unwilling
to take appropriate contraceptive measures during and for at least 6 months after
cessation of therapy

- Patients considered for an autologous or allogenic stem transplant at time of primary
treatment failure or relapse according to the rules of the respective centers

- Any uncontrolled serious non malignant condition or infection which would likely
compromise the study objectives

- Previous evolutive malignancy within 5 years of study entry, with the exception of
non-melanoma skin tumors or stage 0 (in situ) cervical carcinoma

- Major surgery within 4 weeks prior to enrollment, unless patient has recovered from
all treatment related toxicity

- Patient under tutelage.

Type of Study:


Study Design:

Allocation: Non-Randomized, Endpoint Classification: Safety/Efficacy Study, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Treatment

Outcome Measure:

Primary objective:

Principal Investigator

Franck Morschhauser, MD

Investigator Role:

Principal Investigator

Investigator Affiliation:

Lymphoma Study Association


France: Afssaps - Agence française de sécurité sanitaire des produits de santé (Saint-Denis)

Study ID:

RFM Follicular lymphoma study



Start Date:

April 2001

Completion Date:

December 2006

Related Keywords:

  • Follicular Lymphoma
  • relapsed/refractory.
  • lymphoma, follicular
  • rituximab
  • chemotherapy
  • Lymphoma
  • Lymphoma, Follicular
  • Lymphoma, Non-Hodgkin