Molecular Mechanism of Trophinin in Cancer Invasion and Metastasis
This study was to combine the in silico biology and empirical methods to identify clinically
useful biomarker of lung cancer from microarray gene expression profiles in public database.
Using the expression profile of a known metastasis suppressor, CRMP-1, as a template to
search for and compare transcriptome expressions in publicly accessible microarray datasets,
trophinin (TRO) was identified as the best correlated gene. The expression pattern of
trophinin in a lung cancer cell lines of invasion model was investigated using quantitative
RT-PCR. The role of trophinin in cancer metastasis was investigated by measuring the change
of invasion activity in trophinin gene-transfected cell lines. The relationship of trophinin
and the clinicopathological characteristics of lung cancer were further examined by
Additional Descriptors: Convenience Sample, Observational Model: Natural History, Time Perspective: Cross-Sectional, Time Perspective: Retrospective
Kuan-Yu Chen, M.D.
National Taiwan University Hospital
Taiwan: Department of Health