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Clinical Usefulness of Optical Skin Biopsy

Phase 1
Open (Enrolling)
Skin Neoplasms

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Trial Information

Clinical Usefulness of Optical Skin Biopsy

Traditional biopsy requires the removal, fixation, and staining of tissues from the human
body. Its procedure is invasive and painful. Non-invasive in vivo optical biopsy is thus
required, which should provide non-invasive, highly penetrative, three-dimensional (3D)
imaging with sub-micron spatial resolution. Optical biopsy based on scanning two-photon
fluorescence microscopy (TPFM) is a good method for biopsy of skin due to its high lateral
resolution, low out-of-focus damage, and intrinsic three-dimensional (3D) section
capability. However current technology still presents several limitations including low
penetration depth, in-focus cell damages, and multi-photon phototoxicity due to high optical
intensity in the 800 nm wavelength region, and toxicity if exogenous fluorescence markers
were required. We study the harmonics optical biopsy of a human skin sample using a
femtosecond Cr:forsterite laser centered at 1230 nm. Higher harmonics generation is known to
leave no energy deposition to the interacted matters due to their energy-conservation
characteristic. This energy-conservation characteristic provides the “noninvasive” nature
desirable for clinical imaging. In our study, we will evaluate the clinical applications of
optical skin biopsy using harmonic generation microscopy.

Inclusion Criteria:

- Skin lesions admitted for surgical resection.

Exclusion Criteria:

- none

Type of Study:


Study Design:

Observational Model: Defined Population, Observational Model: Natural History, Time Perspective: Cross-Sectional, Time Perspective: Retrospective/Prospective

Principal Investigator

Wen-jeng Lee, M.D.

Investigator Role:

Study Director

Investigator Affiliation:

National Taiwan University Hospital


Taiwan: Department of Health

Study ID:




Start Date:

January 2004

Completion Date:

December 2007

Related Keywords:

  • Skin Neoplasms
  • Neoplasms
  • Skin Neoplasms