Gene Expression Profiling of Infant Embryonal Central Nervous System Tumors by Microarray Gene Chip Analysis: Angiogenesis, Invasion and Metastasis
- Identify known genes that have significantly different levels of expression, using
microarray gene chip analysis, in infants with newly diagnosed metastatic vs
non-metastatic embryonal central nervous system tumors.
- Determine the protein expression of genes identified by microarray analysis that are
involved in cellular functions that regulate angiogenesis, invasion, or metastasis in
this patient population.
- Determine the quantity of gene expression of the confirmed translationally expressed
genes using semi-quantitative polymerase chain reaction.
OUTLINE: This is a multicenter study.
Tumor samples are analyzed using microarray gene chip analysis. Differentially expressed
genes are evaluated for protein expression by standard immunohistochemistry and/or Western
blot analysis, and gene expression is further quantified by semi-quantitative polymerase
PROJECTED ACCRUAL: Approximately 80-100 patients (20-25 with metastatic disease and 60-75
with non-metastatic disease) will be accrued for this study within 4-5 years.
Observational Model: Cohort, Time Perspective: Prospective
Genes that are expressed in metastatic vs. non-metastatic tumors
Prior to therapy
Tobey MacDonald, MD
Children's Research Institute
United States: Federal Government
|Baylor College of Medicine||Houston, Texas 77030|
|Children's Hospital of Philadelphia||Philadelphia, Pennsylvania 19104|
|Duke Comprehensive Cancer Center||Durham, North Carolina 27710|
|UCSF Cancer Center and Cancer Research Institute||San Francisco, California 94115-0128|
|Dana-Farber Cancer Institute||Boston, Massachusetts 02115|
|Children's National Medical Center||Washington, District of Columbia 20010-2970|
|Children's Hospital of Pittsburgh||Pittsburgh, Pennsylvania 15213|
|Children's Hospital and Regional Medical Center - Seattle||Seattle, Washington 98105|
|Saint Jude Children's Research Hospital||Memphis, Tennessee 38105-2794|