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Molecular Biology of Pediatric Brain Tumors

20 Years
Not Enrolling
Brain and Central Nervous System Tumors

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Trial Information

Molecular Biology of Pediatric Brain Tumors


- Determine the chromosomal gains and losses by DNA ploidy analysis and comparative
genomic hybridization in patients with primitive neuroectodermal tumors or

- Determine the frequency of specific chromosomal abnormalities, including deletions of
chromosomal regions 6, 17, and 22, in these patients.

- Perform a statistical analysis to determine possible associations of chromosomal
abnormalities and DNA ploidy with patient age, tumor histology, tumor location, extent
of disease, and event-free survival.

OUTLINE: DNA ploidy analysis will be performed to determine the overall level of aneuploidy.
The results are compared to the comparative genomic hybridization (CGH) analysis, which is
used to demonstrate tumor-specific losses or gains, including amplification, of specific
chromosomal regions. Tumors are also screened for specific abnormalities by fluorescent in
situ hybridization (FISH), which detects chromosomal rearrangements, including balanced
translocations, deletions, amplifications, etc. PCR-based microsatellite polymorphism
analysis may also be performed.

Primitive neuroectodermal tumors (PNETs) are screened by FISH with a distal 17p13.3 cosmid
and a 17q25 cosmid to identify tumors with a 17p deletion. Atypical teratoid/rhabdoid tumors
and PNETs without a 17p deletion are screened by FISH with a series of cosmids from 22q11.2.
PNETs are also screened by interphase FISH with cosmids from chromosome 6 to identify tumors
with deletions.

Patients do not receive the results of the genetic testing and the results do not influence
the type or duration of treatment.

PROJECTED ACCRUAL: This study will accrue 360 specimens.

Inclusion Criteria


- Histologically confirmed primary CNS malignancy consistent with primitive
neuroectodermal tumor, medulloblastoma, or atypical teratoid/rhabdoid tumor

- Must be entered on CCG-9921, CCG-9931, CCG-A9961, CCG-99703 or other front-line
studies developed from CCG-90024 or CCG-90025

- Retrospective specimens also obtained from CCG-921, CCG-923, CCG-9892, CCG-9921, and



- Under 21

Performance status:

- See Disease Characteristics

Life expectancy:

- See Disease Characteristics


- See Disease Characteristics


- See Disease Characteristics


- See Disease Characteristics


Biologic therapy:

- Not specified


- Not specified

Endocrine therapy

- Not specified


- No prior radiotherapy


- Not specified

Type of Study:


Study Design:


Principal Investigator

Jaclyn A. Biegel, PhD

Investigator Role:

Study Chair

Investigator Affiliation:

Children's Hospital of Philadelphia


United States: Federal Government

Study ID:




Start Date:

December 1997

Completion Date:

Related Keywords:

  • Brain and Central Nervous System Tumors
  • untreated childhood supratentorial primitive neuroectodermal tumor
  • untreated childhood medulloblastoma
  • Brain Neoplasms
  • Nervous System Neoplasms
  • Central Nervous System Neoplasms



Children's Hospital of Philadelphia Philadelphia, Pennsylvania  19104
CCOP - Kalamazoo Kalamazoo, Michigan  49007-3731
Carole and Ray Neag Comprehensive Cancer Center at the University of Connecticut Health Center Farmington, Connecticut  06360-2875
CCOP - MeritCare Hospital Fargo, North Dakota  58122
Avera Cancer Institute Sioux Falls, South Dakota  57105
University of Wisconsin Paul P. Carbone Comprehensive Cancer Center Madison, Wisconsin  53792-6164
Marshfield Clinic - Marshfield Center Marshfield, Wisconsin  54449
Newark Beth Israel Medical Center Newark, New Jersey  07112
Deaconess Medical Center Spokane, Washington  99210-0248
Holden Comprehensive Cancer Center at University of Iowa Iowa City, Iowa  52242-1002
Lineberger Comprehensive Cancer Center at University of North Carolina - Chapel Hill Chapel Hill, North Carolina  27599-7570
Cleveland Clinic Taussig Cancer Center Cleveland, Ohio  44195
Monmouth Medical Center Long Branch, New Jersey  07740-6395
Children's Hospital of Orange County Orange, California  92668
Children's National Medical Center Washington, District of Columbia  20010-2970
Children's Mercy Hospital Kansas City, Missouri  64108
Phoenix Children's Hospital Phoenix, Arizona  85016-7710
Southern California Permanente Medical Group Downey, California  90242
Children's Hospital Central California Madera, California  93638-8762
Kosair Children's Hospital Louisville, Kentucky  40202-3830
East Tennessee Children's Hospital Knoxville, Tennessee  37901
Overlook Hospital Summit, New Jersey  07902-0220
Carol G. Simon Cancer Center at Morristown Memorial Hospital Morristown, New Jersey  07962
Loma Linda University Cancer Institute at Loma Linda University Medical Center Loma Linda, California  92354
Jonathan Jaques Children's Cancer Center at Miller Children's Hospital Long Beach, California  90801
Santa Barbara Cottage Children's Hospital Santa Barbara, California  93105
St. Vincent Indianapolis Hospital Indianapolis, Indiana  46260
Blank Children's Hospital Des Moines, Iowa  50309
Breslin Cancer Center at Ingham Regional Medical Center Lansing, Michigan  48910
Cincinnati Children's Hospital Medical Center Cincinnati, Ohio  45229-3039
Rainbow Babies and Children's Hospital Cleveland, Ohio  44106-5000
Texas Tech University Health Sciences Center School of Medicine - Amarillo Amarillo, Texas  79106
Methodist Children's Hospital of South Texas San Antonio, Texas  78229-3993
Primary Children's Medical Center Salt Lake City, Utah  84113-1100
Samuel Oschin Comprehensive Cancer Institute at Cedars-Sinai Medical Center Los Angeles, California  90048-1865
Jonsson Comprehensive Cancer Center at UCLA Los Angeles, California  90095-1781
Children's Hospital Center for Cancer and Blood Disorders Aurora, Colorado  80045
Alfred I. duPont Hospital for Children Wilmington, Delaware  19803
Lombardi Comprehensive Cancer Center at Georgetown University Medical Center Washington, District of Columbia  20007
AFLAC Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta - Egleston Campus Atlanta, Georgia  30322
Curtis and Elizabeth Anderson Cancer Institute at Memorial Health University Medical Center Savannah, Georgia  31403-3089
Advocate Lutheran General Cancer Care Center Park Ridge, Illinois  60068-1174
Swedish-American Regional Cancer Center Rockford, Illinois  61104-2315
Simmons Cooper Cancer Institute Springfield, Illinois  62794-9677
Butterworth Hospital at Spectrum Health Grand Rapids, Michigan  49503-2560
Masonic Cancer Center at University of Minnesota Minneapolis, Minnesota  55455
Children's Hospitals and Clinics of Minnesota - Minneapolis Minneapolis, Minnesota  55404
Children's Hospitals and Clinics of Minnesota - St. Paul St. Paul, Minnesota  55102-2392
CCOP - Nevada Cancer Research Foundation Las Vegas, Nevada  89109-2306
Nationwide Children's Hospital Columbus, Ohio  43205-2696
Dayton Children's - Dayton Dayton, Ohio  45404-1815
Tod Children's Hospital Youngstown, Ohio  44501
Penn State Children's Hospital Hershey, Pennsylvania  17033-0850
Children's Hospital of Pittsburgh of UPMC Pittsburgh, Pennsylvania  15213
Dell Children's Medical Center of Central Texas Austin, Texas  78723
Gundersen Lutheran Center for Cancer and Blood La Crosse, Wisconsin  54601