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Molecular Biology of Pediatric Brain Tumors

20 Years
Not Enrolling
Brain and Central Nervous System Tumors

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Trial Information

Molecular Biology of Pediatric Brain Tumors


- Determine the chromosomal gains and losses by DNA ploidy analysis and comparative
genomic hybridization in patients with primitive neuroectodermal tumors or

- Determine the frequency of specific chromosomal abnormalities, including deletions of
chromosomal regions 6, 17, and 22, in these patients.

- Perform a statistical analysis to determine possible associations of chromosomal
abnormalities and DNA ploidy with patient age, tumor histology, tumor location, extent
of disease, and event-free survival.

OUTLINE: DNA ploidy analysis will be performed to determine the overall level of aneuploidy.
The results are compared to the comparative genomic hybridization (CGH) analysis, which is
used to demonstrate tumor-specific losses or gains, including amplification, of specific
chromosomal regions. Tumors are also screened for specific abnormalities by fluorescent in
situ hybridization (FISH), which detects chromosomal rearrangements, including balanced
translocations, deletions, amplifications, etc. PCR-based microsatellite polymorphism
analysis may also be performed.

Primitive neuroectodermal tumors (PNETs) are screened by FISH with a distal 17p13.3 cosmid
and a 17q25 cosmid to identify tumors with a 17p deletion. Atypical teratoid/rhabdoid tumors
and PNETs without a 17p deletion are screened by FISH with a series of cosmids from 22q11.2.
PNETs are also screened by interphase FISH with cosmids from chromosome 6 to identify tumors
with deletions.

Patients do not receive the results of the genetic testing and the results do not influence
the type or duration of treatment.

PROJECTED ACCRUAL: This study will accrue 360 specimens.

Inclusion Criteria


- Histologically confirmed primary CNS malignancy consistent with primitive
neuroectodermal tumor, medulloblastoma, or atypical teratoid/rhabdoid tumor

- Must be entered on CCG-9921, CCG-9931, CCG-A9961, CCG-99703 or other front-line
studies developed from CCG-90024 or CCG-90025

- Retrospective specimens also obtained from CCG-921, CCG-923, CCG-9892, CCG-9921, and



- Under 21

Performance status:

- See Disease Characteristics

Life expectancy:

- See Disease Characteristics


- See Disease Characteristics


- See Disease Characteristics


- See Disease Characteristics


Biologic therapy:

- Not specified


- Not specified

Endocrine therapy

- Not specified


- No prior radiotherapy


- Not specified

Type of Study:


Study Design:


Principal Investigator

Jaclyn A. Biegel, PhD

Investigator Role:

Study Chair

Investigator Affiliation:

Children's Hospital of Philadelphia


United States: Federal Government

Study ID:




Start Date:

December 1997

Completion Date:

Related Keywords:

  • Brain and Central Nervous System Tumors
  • untreated childhood supratentorial primitive neuroectodermal tumor
  • untreated childhood medulloblastoma
  • Brain Neoplasms
  • Nervous System Neoplasms
  • Central Nervous System Neoplasms



Children's Hospital of PhiladelphiaPhiladelphia, Pennsylvania  19104
CCOP - KalamazooKalamazoo, Michigan  49007-3731
Carole and Ray Neag Comprehensive Cancer Center at the University of Connecticut Health CenterFarmington, Connecticut  06360-2875
CCOP - MeritCare HospitalFargo, North Dakota  58122
Avera Cancer InstituteSioux Falls, South Dakota  57105
University of Wisconsin Paul P. Carbone Comprehensive Cancer CenterMadison, Wisconsin  53792-6164
Marshfield Clinic - Marshfield CenterMarshfield, Wisconsin  54449
Newark Beth Israel Medical CenterNewark, New Jersey  07112
Deaconess Medical CenterSpokane, Washington  99210-0248
Holden Comprehensive Cancer Center at University of IowaIowa City, Iowa  52242-1002
Lineberger Comprehensive Cancer Center at University of North Carolina - Chapel HillChapel Hill, North Carolina  27599-7570
Cleveland Clinic Taussig Cancer CenterCleveland, Ohio  44195
Monmouth Medical CenterLong Branch, New Jersey  07740-6395
Children's Hospital of Orange CountyOrange, California  92668
Children's National Medical CenterWashington, District of Columbia  20010-2970
Children's Mercy HospitalKansas City, Missouri  64108
Phoenix Children's HospitalPhoenix, Arizona  85016-7710
Southern California Permanente Medical GroupDowney, California  90242
Children's Hospital Central CaliforniaMadera, California  93638-8762
Kosair Children's HospitalLouisville, Kentucky  40202-3830
East Tennessee Children's HospitalKnoxville, Tennessee  37901
Overlook HospitalSummit, New Jersey  07902-0220
Carol G. Simon Cancer Center at Morristown Memorial HospitalMorristown, New Jersey  07962
Loma Linda University Cancer Institute at Loma Linda University Medical CenterLoma Linda, California  92354
Jonathan Jaques Children's Cancer Center at Miller Children's HospitalLong Beach, California  90801
Santa Barbara Cottage Children's HospitalSanta Barbara, California  93105
St. Vincent Indianapolis HospitalIndianapolis, Indiana  46260
Blank Children's HospitalDes Moines, Iowa  50309
Breslin Cancer Center at Ingham Regional Medical CenterLansing, Michigan  48910
Cincinnati Children's Hospital Medical CenterCincinnati, Ohio  45229-3039
Rainbow Babies and Children's HospitalCleveland, Ohio  44106-5000
Texas Tech University Health Sciences Center School of Medicine - AmarilloAmarillo, Texas  79106
Methodist Children's Hospital of South TexasSan Antonio, Texas  78229-3993
Primary Children's Medical CenterSalt Lake City, Utah  84113-1100
Samuel Oschin Comprehensive Cancer Institute at Cedars-Sinai Medical CenterLos Angeles, California  90048-1865
Jonsson Comprehensive Cancer Center at UCLALos Angeles, California  90095-1781
Children's Hospital Center for Cancer and Blood DisordersAurora, Colorado  80045
Alfred I. duPont Hospital for ChildrenWilmington, Delaware  19803
Lombardi Comprehensive Cancer Center at Georgetown University Medical CenterWashington, District of Columbia  20007
AFLAC Cancer Center and Blood Disorders Service of Children's Healthcare of Atlanta - Egleston CampusAtlanta, Georgia  30322
Curtis and Elizabeth Anderson Cancer Institute at Memorial Health University Medical CenterSavannah, Georgia  31403-3089
Advocate Lutheran General Cancer Care CenterPark Ridge, Illinois  60068-1174
Swedish-American Regional Cancer CenterRockford, Illinois  61104-2315
Simmons Cooper Cancer InstituteSpringfield, Illinois  62794-9677
Butterworth Hospital at Spectrum HealthGrand Rapids, Michigan  49503-2560
Masonic Cancer Center at University of MinnesotaMinneapolis, Minnesota  55455
Children's Hospitals and Clinics of Minnesota - MinneapolisMinneapolis, Minnesota  55404
Children's Hospitals and Clinics of Minnesota - St. PaulSt. Paul, Minnesota  55102-2392
CCOP - Nevada Cancer Research FoundationLas Vegas, Nevada  89109-2306
Nationwide Children's HospitalColumbus, Ohio  43205-2696
Dayton Children's - DaytonDayton, Ohio  45404-1815
Tod Children's HospitalYoungstown, Ohio  44501
Penn State Children's HospitalHershey, Pennsylvania  17033-0850
Children's Hospital of Pittsburgh of UPMCPittsburgh, Pennsylvania  15213
Dell Children's Medical Center of Central TexasAustin, Texas  78723
Gundersen Lutheran Center for Cancer and BloodLa Crosse, Wisconsin  54601